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Ionic currents in neuroblastoma clone E-7 cells.

L Vyklický·J Michl·V Vlachová·F Vyskocil
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Abstract

Ionic currents were studied in exponentially growing neuroblastoma cells (clone E-7) derived from mouse neuroblastoma C-1300 with the patch-clamp technique in the whole cell mode (Pflügers Arch., 391 (1981) 85-100). In differentiated cells, with one or several processes, an early inactivating inward current approximately equal to 50 microA/cm2 was observed in response to depolarizing steps from the holding potential -60 to -70 mV, which was insensitive to 2 microM tetrodotoxin but readily blocked by Co2+ (6 mM). This inward current was followed by a delayed outward current which was eliminated by 12 mM tetraethylammonium. In the undifferentiated cells, only delayed outward current was observed. It is suggested that, in differentiated cells both Ca2+ and delayed rectifier K+ channels exist, while only the latter are present in undifferentiated cells.

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AnimalsCalciumClone CellsCobaltIon ChannelsMembrane PotentialsMiceNeuroblastomaPotassiumSodiumTetraethylammoniumTetraethylammonium CompoundsTetrodotoxinComparative StudyJournal Article

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L Vyklický, J Michl, V Vlachová, F Vyskocil. 1985-04-09. Ionic currents in neuroblastoma clone E-7 cells.. https://doi.org/10.1016/0304-3940(85)90019-9

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