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Competitive protein binding assay for piritrexim.

J L Woolley·J L Ringstad·C W Sigel
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Abstract

A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by adsorption to dextran-coated charcoal. Piritrexim is measurable over a range of 0.01 to 10.0 micrograms/mL in plasma with a coefficient of variation less than 15%. The limit of sensitivity of the assay is approximately 2 ng/mL. An excellent correlation between this assay and a previously published HPLC method was found. published HPLC method was found.

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Antineoplastic AgentsBinding, CompetitiveChromatography, High Pressure LiquidChromatography, Thin LayerFolic Acid AntagonistsHumansIodine RadioisotopesProtein BindingPyrimidinesJournal Article

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J L Woolley, J L Ringstad, C W Sigel. 1989. Competitive protein binding assay for piritrexim.. https://doi.org/10.1002/jps.2600780910

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