Taipoxin-binding protein on synaptic membranes: identification by affinity labeling.

M C Tzeng; M J Hseu; C H Yen

doi:10.1016/s0006-291x(89)80021-x

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PubMed · 2597156

Taipoxin-binding protein on synaptic membranes: identification by affinity labeling.

M C Tzeng·M J Hseu·C H Yen

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Abstract

Affinity labeling techniques were used to identify the neuronal membrane molecules involved in the binding of taipoxin, a neurotoxic protein with phospholipase A2 activity. After [125I]taipoxin had bound to synaptosomes from guinea pig brain, treatment with disuccinimidyl suberate resulted in the formation of a predominant radioactive conjugate of 60,000 Da. Notexin and some other PLA2s are weakly inhibitory to this conjugation, while beta-bungarotoxin and some others are not inhibitory. The 60K conjugate was not detected when plasma membranes from several nonneuronal tissues were used. We concluded that a 45,000 Da protein specifically present in neuronal membranes is (a subunit of) the major molecule responsible for taipoxin binding.

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Affinity Labels Animals Brain Carrier Proteins Elapid Venoms Guinea Pigs Molecular Weight Neuromuscular Blocking Agents Phospholipases A Phospholipases A2 Succinimides Synaptic Membranes Synaptosomes Journal Article Research Support, Non-U.S. Gov't

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M C Tzeng, M J Hseu, C H Yen. 1989-12-15. Taipoxin-binding protein on synaptic membranes: identification by affinity labeling.. https://doi.org/10.1016/s0006-291x(89)80021-x

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