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Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions.

E Holmberg·K Maruyama·D C Litzinger·S Wright·M Davis·G W Kabalka·S J Kennel·L Huang
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Abstract

Monoclonal antibody was conjugated to N-glutaryl-phosphatidylethanolamine in the presence of octylglucoside by using N-hydroxysulfosuccinimide as a carboxyl-activation reagent. The conjugated antibody was then incorporated into liposomes by a simple dialysis method. The method is mild and is compatible with various lipid compositions of the liposomes. We have prepared immunoliposomes containing a lung endothelium-specific monoclonal antibody and showed excellent target binding (approximately 75% injected dose) of the immunoliposomes in mouse. Immunoliposomes can be prepared to contain other acidic lipids such as phosphatidylserine and various amounts of cholesterol. The presence of 20% or more cholesterol in liposomes resulted in high level of target binding. We have used in these experiments a new radioactive lipid-phase marker, 111In-DTPA-SA, which was very stable in vivo. The halflife of clearance in mouse exceeded 3 weeks.

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AnimalsAntibodies, MonoclonalCholesterolEndotheliumIndium RadioisotopesKineticsLiposomesLungMiceMice, Inbred BALB CPentetic AcidPhosphatidylcholinesPhosphatidylethanolaminesPhosphatidylserinesRatsTissue DistributionJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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E Holmberg, K Maruyama, D C Litzinger, S Wright, M Davis, G W Kabalka, S J Kennel, L Huang. 1989-12-29. Highly efficient immunoliposomes prepared with a method which is compatible with various lipid compositions.. https://doi.org/10.1016/0006-291x(89)92740-x

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