Three-dimensional imaging in fluorescence by confocal scanning microscopy.

G J Brakenhoff; H T van der Voort; E A van Spronsen; N Nanninga

doi:10.1111/j.1365-2818.1989.tb00555.x

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Three-dimensional imaging in fluorescence by confocal scanning microscopy.

G J Brakenhoff·H T van der Voort·E A van Spronsen·N Nanninga

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Abstract

The improved resolution and sectioning capability of a confocal microscope make it an ideal instrument for extracting three-dimensional information especially from extended biological specimens. The imaging properties, also with finite detection pinholes are considered and a number of biological applications demonstrated.

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Animals Chlamydomonas Image Processing, Computer-Assisted Lasers Mathematics Microscopy, Fluorescence Neoplastic Stem Cells Plants Saccharomyces cerevisiae Tumor Cells, Cultured Journal Article Research Support, Non-U.S. Gov't

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G J Brakenhoff, H T van der Voort, E A van Spronsen, N Nanninga. 1989. Three-dimensional imaging in fluorescence by confocal scanning microscopy.. https://doi.org/10.1111/j.1365-2818.1989.tb00555.x

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