Immunophenotyping in a multicenter study: the Transfusion Safety Study experience.

M A Fletcher; S P Azen; B Adelsberg; G Gjerset; J Hassett; J Kaplan; J C Niland; T Odom-Maryon; J W Parker; D P Stites

doi:10.1016/0090-1229(89)90191-8

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Immunophenotyping in a multicenter study: the Transfusion Safety Study experience.

M A Fletcher·S P Azen·B Adelsberg·G Gjerset·J Hassett·J Kaplan·J C Niland·T Odom-Maryon·J W Parker·D P Stites

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Abstract

The Transfusion Safety Study (TSS) is a cooperative investigation of factors that determine the occurrence of and modify the expression of transfusion-transmitted infections. A major component of its data is derived from lymphocyte immunophenotyping using a large panel of monoclonal antibodies and two-color flow cytometric analysis. The multicenter longitudinal character of TSS necessitates a uniformity of instrumentation, reagents, and protocols, as well as an intensive quality control program. The baseline assessment of a cohort of males 10 years of age and over with congenital clotting disorders (CCD) exemplifies the approach and some of the flow cytometry results. A comparison of anti-HIV-1 positive and negative subjects shows that more of the loss of T4+ cells was attributable to a decrease in the T4+4B4+ subset than the T4+2H4+ subset. There was an overall increase in CD8 cells, with a significant increase in the I2+T8+ and Leu7+T8+ cells, but a fall in NKH.1+T8+ cells. Monocytes, MO2+I2+ cells, increased. In CCD patients under the age of 10, both anti-HIV-1 positive and negative, there were absolute elevations in immunocytes, including CD4. There was also a distinctly different distribution of CD4 subsets. The suppressor inducer subset, 2H4+T4+, was increased relative to the helper inducer subset, 4B4+T4+, in the younger subjects.

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Acquired Immunodeficiency Syndrome Blood Coagulation Disorders Child Communicable Disease Control Flow Cytometry HIV-1 Humans Longitudinal Studies Lymphocytes Male Multicenter Studies as Topic Phenotype Transfusion Reaction Clinical Trial Journal Article Multicenter Study Research Support, U.S. Gov't, P.H.S.

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M A Fletcher, S P Azen, B Adelsberg, G Gjerset, J Hassett, J Kaplan, J C Niland, T Odom-Maryon, J W Parker, D P Stites. 1989. Immunophenotyping in a multicenter study: the Transfusion Safety Study experience.. https://doi.org/10.1016/0090-1229(89)90191-8

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