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Fetal blood sampling.

F Daffos
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Abstract

Fetal blood sampling under ultrasound guidance has permitted the opening of the fetal vascular compartment for diagnosis and therapy. Under very strict conditions, this procedure allows the clinician to use fetal blood analysis as a complementary routine examination.

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Blood Specimen CollectionFemaleFetal BloodFetal DiseasesHumansPregnancyPrenatal DiagnosisUltrasonographyJournal ArticleReview

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BibTeXRIS

F Daffos. 1989. Fetal blood sampling.. https://doi.org/10.1146/annurev.me.40.020189.001535

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Therapeutic drug monitoring of tacrolimus with the dried blood spot method.

In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was <7.5% and 15%, respectively. Tacrolimus concentrations of 24 stable out patients were compared after both blood spot sampling and conventional venous sampling. Method agreement was investigated with the methods of Passing and Bablok and Bland Altman and proved suitable for clinical use. The dried blood spot method for tacrolimus seems promising for patient monitoring.

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