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Desmin-containing intermediate filaments: structural analysis using monoclonal antibodies.

M J Allen·P Johnson
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Abstract

1. Monoclonal antibodies have been raised against N- and C-terminal desmin sequence regions. 2. The antibodies decorated desmin intermediate filaments in a helical fashion with four antibody molecules per helix turn. 3. The filaments could be decorated with both types of antibody consecutively. 4. These results support a model of intermediate filament assembly in which the tetrameric protofilaments are aligned in a staggered fashion with partial overlapping of the central rod domain regions of the desmin sequence.

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AnimalsAntibodies, MonoclonalChickensCytoskeletonDesminImmunologic TechniquesIntermediate FilamentsMicroscopy, ElectronMolecular StructureJournal Article

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M J Allen, P Johnson. 1989. Desmin-containing intermediate filaments: structural analysis using monoclonal antibodies.. https://doi.org/10.1016/0020-711x(89)90215-2

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