Yeast proteins that recognize nuclear localization sequences.

P Silver; I Sadler; M A Osborne

doi:10.1083/jcb.109.3.983

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Yeast proteins that recognize nuclear localization sequences.

P Silver·I Sadler·M A Osborne

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Abstract

A variety of peptides can mediate the localization of proteins to the nucleus. We have identified yeast proteins of 70 and 59 kD that bind to nuclear localization peptides of SV-40 T antigen, Xenopus nucleoplasmin, and the yeast proteins Ga14 and histone H2B. These proteins are assayed by the binding of peptide-albumin conjugates to proteins immobilized on nitrocellulose filters. These binding proteins fractionate with nuclei and are extractable with salt but not detergent. Radiolabeled peptide-albumin conjugates also bind to isolated nuclei; the binding is saturable and can be extracted with salt. Different nuclear localization peptides compete with each other, implying that a single class of proteins is responsible for their recognition. The 70- and 59-kD proteins have the properties expected for a receptor that would act to direct proteins to the nucleus.

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Amino Acid Sequence Animals Antigens, Viral, Tumor Cell Nucleus Cytoplasm Fungal Proteins Kinetics Molecular Sequence Data Molecular Weight Nuclear Proteins Protein Binding Saccharomyces cerevisiae Xenopus Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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P Silver, I Sadler, M A Osborne. 1989. Yeast proteins that recognize nuclear localization sequences.. https://doi.org/10.1083/jcb.109.3.983

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