Biospecific interactions: their quantitative characterization and use for solute purification.

D J Winzor; J De Jersey

doi:10.1016/s0378-4347(00)84476-x

Home/Archive/PubMed

PubMed · 2670999

Biospecific interactions: their quantitative characterization and use for solute purification.

D J Winzor·J De Jersey

View original source ↗Export metadata ↓

Abstract

Biospecificity is due largely to the formation and dissociation of non-covalent complexes between small molecules and macromolecules, or between two macromolecules. The first part of this review is concerned with the use of such biospecificity in the fractionation and identification of solutes. Major emphasis is given to affinity chromatography, especially in regard to the practical considerations inherent in an experimental situation and to the wide range of specific interactions that can be utilized. The second part of the review considers the quantitative characterization of biospecific complex formation. The merits of frontal gel chromatography, electrophoretic methods and affinity chromatography are discussed, and special consideration is given to the effects of ligand and/or acceptor multivalency because of its relevance to many biospecific interactions. Finally attention is drawn to the feasibility of employing quantitative affinity chromatographic theory for the determination of association constants for antigen-antibody systems by radioimmunoassay and enzyme-linked immunosorbent assay techniques.

Explore related subjects

Biochemistry Chromatography, Affinity Solubility Solvents Journal Article Review

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

D J Winzor, J De Jersey. 1989-08-11. Biospecific interactions: their quantitative characterization and use for solute purification.. https://doi.org/10.1016/s0378-4347(00)84476-x

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2025

Molecular differences between young and mature stria vascularis from organotypic explants and transcriptomics.

The stria vascularis (SV) is an essential component of the inner ear that regulates the ionic environment required for hearing. SV degeneration disrupts cochlear homeostasis, leading to irreversible hearing loss, yet a comprehensive understanding of the SV, and consequently therapeutic availability for SV degeneration, is lacking. We developed a whole-tissue explant model from neonatal and mature mice to create a platform for advancing SV research. We validated our model by demonstrating that the proliferative behavior of the SV in vitro mimics SV in vivo. We also provided evidence for pharmacological experimentation by investigating the role of Wnt/β-catenin signaling in SV proliferation. Finally, we performed single-cell RNA sequencing from in vivo neonatal and mature mouse SV and surrounding tissue and revealed key genes and pathways that may play a role in SV proliferation and maintenance. Together, our results contribute new insights into investigating biological solutions for SV-associated hearing loss.

Biochemistry↗

PubMed2007

Manipulation of cell-sized phospholipid-coated microdroplets and their use as biochemical microreactors.

Cell-sized water droplets coated by a phospholipid layer mimicking the inner surface of living cells were manipulated by laser tweezers and used as biochemical microreactors. The cell-sized phospholipid-coated microdroplets (CPMDs) consisted of a water droplet in mineral oil with a diameter of 1-100 microm and coated by 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. We monitored the time development of biochemical reactions in a single CPMD obtained after the controlled fusion of two CPMDs containing a substrate and an enzyme, respectively. We present results on two enzymatic reactions: calcein production in the presence of esterase and green fluorescence protein expression.

Biochemistry↗

PubMed2007

Classical versus stochastic kinetics modeling of biochemical reaction systems.

We study fundamental relationships between classical and stochastic chemical kinetics for general biochemical systems with elementary reactions. Analytical and numerical investigations show that intrinsic fluctuations may qualitatively and quantitatively affect both transient and stationary system behavior. Thus, we provide a theoretical understanding of the role that intrinsic fluctuations may play in inducing biochemical function. The mean concentration dynamics are governed by differential equations that are similar to the ones of classical chemical kinetics, expressed in terms of the stoichiometry matrix and time-dependent fluxes. However, each flux is decomposed into a macroscopic term, which accounts for the effect of mean reactant concentrations on the rate of product synthesis, and a mesoscopic term, which accounts for the effect of statistical correlations among interacting reactions. We demonstrate that the ability of a model to account for phenomena induced by intrinsic fluctuations may be seriously compromised if we do not include the mesoscopic fluxes. Unfortunately, computation of fluxes and mean concentration dynamics requires intensive Monte Carlo simulation. To circumvent the computational expense, we employ a moment closure scheme, which leads to differential equations that can be solved by standard numerical techniques to obtain more accurate approximations of fluxes and mean concentration dynamics than the ones obtained with the classical approach.

Biochemistry↗