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PubMed · 2683193

von Willebrand factor multimer analysis using a sensitive peroxidase staining method.

Y Tomita·J Harrison·C F Abildgaard
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Abstract

A peroxidase staining method for von Willebrand factor (vWF) multimer analysis was modified for comparison with an autoradiographic method. This method consists of SDS-agarose electrophoresis followed by blotting to a nitrocellulose membrane and a sensitive peroxidase staining method. The resolution of vWF multimers on the nitrocellulose membrane is comparable to that on the conventional autoradiography. Results can be obtained in 3 days. This nonradioisotopic method will be useful for the determination of the type of von Willebrand's disease in clinical laboratories.

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AutoradiographyBiopolymersElectrophoresis, Agar GelHumansImmunoenzyme Techniquesvon Willebrand FactorJournal Article

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Y Tomita, J Harrison, C F Abildgaard. 1989-09-29. von Willebrand factor multimer analysis using a sensitive peroxidase staining method.. https://pubmed.ncbi.nlm.nih.gov/2683193/

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