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PubMed · 2687609

An automated reticulocyte counting method: preliminary observations.

N Tatsumi·I Tsuda·K Kojima·M Niri·K Setoguchi
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Abstract

We evaluated the counting of reticulocytes in the peripheral blood with a newly developed flow cytometer type of automated counter that performs a single test within 60 s. The volume of sample needed is 100 microliters and the cells are stained with auramine-O in the counter. The mean within-run reproducibility was 4.66% (CV, n = 50), and dilution of blood gave highly linear results with an r value of 0.996. Correlation was good between manual reticulocyte counts and those performed with the counter (r = 0.893). Samples with large numbers of leucocytes, erythrocytes, or platelets did not interfere with the automated reticulocyte counting, and provided accurate and precise data.

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AutoanalysisBlood Cell CountFlow CytometryHumansReticulocytesSensitivity and SpecificityJournal Article

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N Tatsumi, I Tsuda, K Kojima, M Niri, K Setoguchi. 1989. An automated reticulocyte counting method: preliminary observations.. https://pubmed.ncbi.nlm.nih.gov/2687609/

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