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PubMed · 2695072

REP3-derived yeast shuttle vector.

U Schubert·D Gniel·H Lang
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Abstract

A yeast-shuttle vector using a XbaI-PstI fragment of the 2 microns DNA 1293 bp in length has been constructed. This sequence spans the REP 3 locus and the origin of replication. Besides the 2 microns DNA derived sequences the resulting yeast shuttle vector contains the yeast LEU 2 gene and the Tet' gene of pBR 322. The results demonstrate that the XbaI-PstI fragment is sufficient for proper amplification and partitioning of 2 microns DNA derived yeast-shuttle vectors in yeast. The interruption of the FRT site in this type of vector seems to prevent any recombination between endogenous 2 microns DNA and hybrid plasmid molecules. Moreover, the disturbance of the FLP-system has no influence on the copy number of the plasmid stability.

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Blotting, SouthernDNA ReplicationDNA, FungalDensitometryGene AmplificationGenetic VectorsMitosisSaccharomyces cerevisiaeTransformation, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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U Schubert, D Gniel, H Lang. 1989. REP3-derived yeast shuttle vector.. https://pubmed.ncbi.nlm.nih.gov/2695072/

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