Confocal scanning microscopy: three-dimensional biological imaging.

D Shotton; N White

doi:10.1016/0968-0004(89)90096-0

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Confocal scanning microscopy: three-dimensional biological imaging.

D Shotton·N White

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Abstract

Confocal scanning optical microscopy, arguably the most significant in biological light microscopy in this decade, enables one to obtain quantitative non-invasive optical sections through labelled biological specimens, virtually free from out-of-focus blur. A set of these optical sections collected at a series of focal levels through an object constitutes a three-dimensional image which may then be processed digitally for display as a computer reconstruction, a stereo pair or an animation sequence.

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Animals Fibroblasts Image Processing, Computer-Assisted Lung Microscopy Microscopy, Fluorescence Journal Article Research Support, Non-U.S. Gov't Review

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D Shotton, N White. 1989. Confocal scanning microscopy: three-dimensional biological imaging.. https://doi.org/10.1016/0968-0004(89)90096-0

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