Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.

C A Brenner; A W Tam; P A Nelson; E G Engleman; N Suzuki; K E Fry; J W Larrick

PubMed · 2698190

Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.

C A Brenner·A W Tam·P A Nelson·E G Engleman·N Suzuki·K E Fry·J W Larrick

Abstract

A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and enzymatic amplification of cytokine-specific DNA fragments using the polymerase chain reaction. In this study, the technique has been applied to the analysis of cytokines produced by lymphoid cells ranging in number from a single cell to 10(6) cells. The technique should be applicable to virtually any tissue or cell type.

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Base Sequence Biological Factors Biotechnology Cell Line Cytokines DNA Genetic Techniques Humans Kinetics Molecular Sequence Data Phenotype Polymerase Chain Reaction RNA, Messenger Journal Article

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C A Brenner, A W Tam, P A Nelson, E G Engleman, N Suzuki, K E Fry, J W Larrick. Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.. https://pubmed.ncbi.nlm.nih.gov/2698190/

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