[A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].

P Ia Arenkov; E N Zhmareva; V A Berezin

PubMed · 2698529

[A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].

Abstract

The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase. The purified rabbit immunoglobulin G is immobilized on the surface of membranes as the first layer. The competitive immunoassay is employed. The immunoglobulin G concentration range is 1-1000 ng/ml. The membranes with the immobilized antigen can be repeatedly used after incubation in 0.1 M glycine buffer, pH 2.5. The dry membrane with the immobilized antigen can be used after keeping for 6 months in refrigerator at 4 degrees C without changing the concentration range measured.

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Animals Antigens Immunoenzyme Techniques Immunoglobulin G Membranes, Artificial Rabbits English Abstract Journal Article

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P Ia Arenkov, E N Zhmareva, V A Berezin. [A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes].. https://pubmed.ncbi.nlm.nih.gov/2698529/

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