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Surface antigen in early differentiation.

R Kemler·C Babinet·H Eisen·F Jacob
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Abstract

Addition of Fab fragments from rabbit antiserum to surface antigen F9 to 2-cell stage mouse embryos in culture does not alter cleavage; however, the addition prevents culture does not alter cleavage; however, the addition prevents the formation of compact morulae and blastocysts. A similar effect is observed when Fab fragments are added to already compact 8-cell stage or even older morulae, but disappears at the beginning of blastocoel formation. This effect is reversible: uncompact 30-cell embryos washed free of Fab become compact in a few hours, produce blastocysts, and upon reimplantation into pseudopregnant mothers can produce mice. Development is not altered by divalent anti-F9 antibodies, by Fab fragments from sera directed against other embryo surface antigens, or by succinyl concanavalin A.

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AnimalsAntigensBlastocystCell DifferentiationCell LineEmbryo, MammalianFemaleImmunoglobulin Fab FragmentsImmunoglobulin GLymphocytesMiceMice, Inbred C57BLMice, Inbred CBAMice, Inbred StrainsPregnancyRabbitsJournal Article

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R Kemler, C Babinet, H Eisen, F Jacob. 1977. Surface antigen in early differentiation.. https://doi.org/10.1073/pnas.74.10.4449

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