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Quantification of rat T-kininogen using immunological methods. Application to inflammatory processes.

A Adam·J Damas·G Calay·C Renard·G Remacle-Volon·V Bourdon
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Abstract

Antibodies raised in rabbits against rat T-kininogen (alpha 1-cysteine proteinase inhibitor) were used to develop a radioimmunoassay and a nephelometric quantification for T-kininogen. These assays were specific and analytically reliable. We also described a radioimmunoassay for kinin measurement. These immunological methods have been used to study the behaviour of T-kininogen during inflammatory processes and specify the two properties of this kind of kininogen: its inhibitory capacity towards cysteine proteinases and its activity as precursor of T-kinin. Control plasma level of T-kininogen in male rats was lower than that of female rats. The maximum level was observed in plasma, liver, kidney and uterus of female rats during metestrus. After turpentine injection, T-kininogen level increased not only in plasma but also in liver and kidney. In carrageenan-induced peritoneal exudates, we found a large accumulation of T-kininogen and of immunoreactive kinins, these latter being identified by HPLC as bradykinin.

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AnimalsFemaleInflammationKininogensKininsMaleNephelometry and TurbidimetryRadioimmunoassayRatsRats, Inbred StrainsTurpentineJournal ArticleResearch Support, Non-U.S. Gov't

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A Adam, J Damas, G Calay, C Renard, G Remacle-Volon, V Bourdon. 1989-05-15. Quantification of rat T-kininogen using immunological methods. Application to inflammatory processes.. https://doi.org/10.1016/0006-2952(89)90303-1

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