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An efficient laboratory made apparatus for DNA amplification.

O Bertrand·M H Delfau·M Garbarz·C Picat·I Devaux·D Dhermy·P Boivin·B Grandchamp
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Abstract

DNA amplification by the polymerase chain reaction (PCR) is a method capable of producing a selective and very high enrichment of a specific DNA sequence. Hence it seems to be useful in various fields from basic research to clinical applications. In order to automatize PCR we assembled for a very low cost a mechanical system designed to carry a test tube holder successively in three thermal baths set at the required temperatures for the reaction. Two examples of the use of this machine are given: (i) amplification of DNA of a particular subtype of acute intermittent porphyria; (ii) the detection of the chimeric c-abl/bcr message found in chronic myelogenous leukemia cells.

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Base SequenceDNADNA-Directed DNA PolymeraseHumansLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMolecular Sequence DataNucleic Acid Amplification TechniquesPorphyriasProto-OncogenesRNA, MessengerJournal Article

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O Bertrand, M H Delfau, M Garbarz, C Picat, I Devaux, D Dhermy, P Boivin, B Grandchamp. 1989. An efficient laboratory made apparatus for DNA amplification.. https://doi.org/10.1016/0165-022x(89)90007-9

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