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Electrically regulated cellular morphological and cytoskeletal changes on an optically transparent electrode.

M Yaoita·M Aizawa·Y Ikariyama
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Abstract

Electrically regulated morphological and cytoskeletal changes of HeLa cells were studied on an optically transparent electrode (OTE), on which potential-applied surface cellular behavior and morphogenesis were easily observed. Upon application of a potential, HeLa cells in an OTE exhibited remarkable morphological changes above +0.5 V (vs. Ag/AgCl) and below 0 V. At each potential in this potential range, change in F-actin distribution was observed using a fluorescent probe (rhodamine phalloidin). These results suggest that an electrical field induces a subcellular cytoskeletal change. Electrostimulation of cells with OTE can be a valuable strategy for the manipulation of cultured human cells.

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ActinsCell MembraneCytoskeletonElectric StimulationHeLa CellsHumansKineticsMicroelectrodesJournal Article

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M Yaoita, M Aizawa, Y Ikariyama. 1989. Electrically regulated cellular morphological and cytoskeletal changes on an optically transparent electrode.. https://doi.org/10.1159/000163506

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