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PubMed · 2745642

Multi-immunoaffinity chromatography: a simple and highly selective clean-up method for multi-anabolic residue analysis of meat.

L A van Ginkel·R W Stephany·H J van Rossum·H M Steinbuch·G Zomer·E Van de Heeft·A P De Jong
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Abstract

A method for the detection of nortestosterone (NT) in bovine muscle at levels below 1 microgram/kg is described, based on enzymatic digestion of the sample, clean-up by immunoaffinity chromatography after defatting and detection by gas chromatography-mass spectrometry (selected-ion monitoring). The immunoaffinity matrix was prepared after combining the isolated immunoglobulin G fractions from a rabbit antiserum raised against NT and methyltestosterone (MT). Its capacity per millilitre of gel was approximately 10 ng for each of the two steroids. Results for samples containing 0.1 microgram/kg NT and above are described. It is concluded that for multi-residue analysis of samples of muscle at levels as low as 0.1 microgram/kg, multi-immunoaffinity chromatography is a very suitable method of sample clean-up. For purposes of quantification the trideuterated internal standard [16,16,17 alpha-2H3] nortestosterone was synthesized.

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Anabolic AgentsAnimalsCattleChromatography, AffinityDrug ResiduesFood ContaminationGas Chromatography-Mass SpectrometryMeatRadioimmunoassayJournal Article

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L A van Ginkel, R W Stephany, H J van Rossum, H M Steinbuch, G Zomer, E Van de Heeft, A P De Jong. 1989-04-07. Multi-immunoaffinity chromatography: a simple and highly selective clean-up method for multi-anabolic residue analysis of meat.. https://doi.org/10.1016/s0378-4347(00)82888-1

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