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Radiation inactivation analysis of the A1 adenosine receptor of rat brain. Decrease in radiation inactivation size in the presence of guanine nucleotide.

M Reddington·K N Klotz·M J Lohse·B Hietel
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Abstract

Radiation inactivation analysis of the binding of the A1 adenosine receptor antagonist, 8-cyclopentyl-1,3-dipropylxanthine to rat brain membranes yielded a radiation inactivation size of 58 kDa. In the presence of GTP gamma S this was reduced to 33 kDa, in good agreement with the size of the ligand-binding subunit detected after photoaffinity labelling. The data indicate that the structural association of A1 adenosine receptors with G-protein components is altered in situ in the presence of guanine nucleotides.

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AdenosineAnimalsBrainGuanine NucleotidesRatsReceptors, PurinergicXanthinesJournal ArticleResearch Support, Non-U.S. Gov't

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M Reddington, K N Klotz, M J Lohse, B Hietel. 1989-07-31. Radiation inactivation analysis of the A1 adenosine receptor of rat brain. Decrease in radiation inactivation size in the presence of guanine nucleotide.. https://doi.org/10.1016/0014-5793(89)80903-2

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