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Technique for cellular fluorescence distribution analysis.

R D Robinson·J E Reeder·L L Wheeless
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Abstract

The usefulness of multidimensional slit-scan flow cytometry in whole cell measurements is dependent on extracting relevant features from the cellular fluorescence distributions (slit-scan contours). In addition, the extraction of these features must be rapid to allow for real-time data processing during acquisition. This paper describes two algorithms that have been used successfully to count the numbers of local maxima (peaks) and to find nuclear boundaries in a cellular fluorescence distribution. These routines are efficient, use only simple integer arithmetic, and have been implemented on several different microprocessors.

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AlgorithmsFlow CytometryFluorescenceHumansJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R D Robinson, J E Reeder, L L Wheeless. 1989. Technique for cellular fluorescence distribution analysis.. https://doi.org/10.1002/cyto.990100407

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PubMed2026

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