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Algorithm to estimate cell biovolume using image analyzed microscopy.

M E Sieracki·C L Viles·K L Webb
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Abstract

This paper describes an algorithm for calculating the biovolume of cells with simple shapes, such as bacteria, flagellates, and simple ciliates, from a 2-dimensional digital image. The method can be adapted to any image analysis system which allows access to the binary cell image--(i.e., the pixels, or (x,y) points, composing the cell. The cell image is rotated to a standard orientation (horizontal), and a solid of revolution is calculated by digital integration. Verification and a critical assessment of the method are presented. The algorithm accounts for irregularities in cell shape that conventional methods based on length, width, and geometrical formulas do not.

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AlgorithmsBacteriaImage Processing, Computer-AssistedMicroscopy, FluorescenceProkaryotic CellsJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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BibTeXRIS

M E Sieracki, C L Viles, K L Webb. 1989. Algorithm to estimate cell biovolume using image analyzed microscopy.. https://doi.org/10.1002/cyto.990100510

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