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PubMed · 2799384

Detection of cell-affecting agents with a silicon biosensor.

J W Parce·J C Owicki·K M Kercso·G B Sigal·H G Wada·V C Muir·L J Bousse·K L Ross·B I Sikic·H M McConnell
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Abstract

Cellular metabolism is affected by many factors in a cell's environment. Given a sufficiently sensitive method for measuring cellular metabolic rates, it should be possible to detect a wide variety of chemical and physical stimuli. A biosensor has been constructed in which living cells are confined to a flow chamber in which a potentiometric sensor continually measures the rate of production of acidic metabolites. Exploratory studies demonstrate several applications of the device in basic science and technology.

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Biosensing TechniquesCarbonyl Cyanide m-Chlorophenyl HydrazoneCellsCells, CulturedEpidermal Growth FactorFlow CytometryHumansOxygen ConsumptionSiliconJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J W Parce, J C Owicki, K M Kercso, G B Sigal, H G Wada, V C Muir, L J Bousse, K L Ross, B I Sikic, H M McConnell. 1989-10-13. Detection of cell-affecting agents with a silicon biosensor.. https://doi.org/10.1126/science.2799384

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