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PubMed · 2809464

A method for visualizing cytoskeletal structures by the scanning electron microscope.

I Takahashi·Y Amakawa
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Abstract

The cytoskeleton of intestinal epithelial cells was observed by scanning electron microscope (SEM). Tissues were treated with Triton X-100, stained to enhance conductivity, and freeze-fractured in liquid nitrogen. With these procedures, not only the localization of core filaments (actin-containing microfilaments) and intermediate filaments but also the relations between fibrous structures and cell organellae were clearly revealed. This conventional method is of great value in interpreting the three-dimensional organization of intracellular fine structures.

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AnimalsCytoskeletonFreeze FracturingIntestine, SmallMaleMiceMicroscopy, Electron, ScanningJournal Article

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I Takahashi, Y Amakawa. 1989. A method for visualizing cytoskeletal structures by the scanning electron microscope.. https://pubmed.ncbi.nlm.nih.gov/2809464/

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