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Stability function in the Bacillus subtilis plasmid pTA 1060.

S Bron·P Bosma·M van Belkum·E Luxen
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Abstract

Plasmid pBB2 (11.3 kb) was constructed by genetically labeling the cryptic Bacillus subtilis plasmid pTA 1060 with the pC194-derived CmR and the pUB110-derived KmR markers. In nonselective media pBB2 was segregationally almost completely stable (loss rates less than or equal to 0.02% per cell generation). In contrast, pBB3, obtained by deleting from pBB2 a region consisting of two ClaI fragments (1.45 and 0.20 kb, respectively), was unstable (loss rates greater than or equal to 0.5% per cell generation). This indicates that a genetic element required for stability is located on one or both of these fragments. In pBB3 cop, a mutant with a two- to threefold increased copy number, the rate of plasmid loss was reduced compared to that of pBB3. The insertion of a 4.2-kb Escherichia coli DNA fragment reduced the stability of pBB2 only slightly, suggesting that this vector may be useful for the cloning of relatively large fragments.

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Bacillus subtilisCloning, MolecularDNA ReplicationDNA Transposable ElementsDNA, BacterialGenotypePlasmidsJournal Article

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S Bron, P Bosma, M van Belkum, E Luxen. 1987. Stability function in the Bacillus subtilis plasmid pTA 1060.. https://doi.org/10.1016/0147-619x(87)90073-4

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