PubMed · 282742
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F Masin, M Masin. Sputum blending.. https://pubmed.ncbi.nlm.nih.gov/282742/
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We describe an efficient method for embedding frozen-section specimens. Transparent tape is used to produce a well on the surface of the tissue holder ("chuck"). This well contains a quantity of nonmotile mounting media in which a tissue specimen can be easily oriented. After freezing, the tape is removed, leaving a relatively smooth surface.
Virocult was used as a supplemental viral collection and transportation system. It compared favorably with tryptose phosphate broth in maintaining clinically important viruses. Advantages of Virocult over tryptose phosphate broth are commercial availability; extended shelf life at ambient temperature; and ease of use.