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PubMed · 2827650

Protein kinase C and parietal cell function.

W Beil·W Mannschedel·K F Sewing
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Abstract

Ca2+-phospholipid dependent protein kinase activity and the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on H+ secretion was studied in guinea-pig parietal cells. In resting parietal cells PK-C activity was distributed almost equally in both the cytosolic and particulate cell fraction. Exposure of the cells to TPA resulted in a loss of cytosolic PK-C activity which was not accompanied by a concomitant increase of particulate activity. Furthermore TPA inhibits the K+/H+-ATPase and dissipates H+ gradient in intact gastric vesicles by a protonophoric action. The role of these biochemical events for the antisecretory action of TPA found in the intact parietal cell are discussed.

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Adenosine TriphosphatasesAnimalsCalciumGastric AcidGuinea PigsH(+)-K(+)-Exchanging ATPaseParietal Cells, GastricProtein Kinase CTetradecanoylphorbol AcetateJournal Article

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W Beil, W Mannschedel, K F Sewing. 1987-12-16. Protein kinase C and parietal cell function.. https://doi.org/10.1016/0006-291x(87)90427-x

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