Genetic organization of insertion element IS2 based on a revised nucleotide sequence.

H J Ronecker; B Rak

doi:10.1016/0378-1119(87)90337-4

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Genetic organization of insertion element IS2 based on a revised nucleotide sequence.

H J Ronecker·B Rak

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Abstract

We identified a transposable element resident in the chromosome of Escherichia coli K-12 strain HB101. This is an approx. 4400-bp-long transposon flanked by two copies of insertion sequence (IS) 1 element in direct orientation. One of the IS1 elements was found to be integrated into an IS2 element between IS2 bp 139 and bp 140 with the large moiety of IS2 within the transposon. The sequence of this part of IS2 differs from the published sequence of galOP-308::IS2 at a number of positions. Restriction analysis of the published allele, however, indicated that both alleles may in fact be identical. Since six of the eight differences found alter open reading frames, the revised sequence presents a new outlook for the potential genetic organization of IS2.

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Base Composition Base Sequence Chromosomes, Bacterial DNA Restriction Enzymes DNA Transposable Elements Escherichia coli Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization Journal Article Research Support, Non-U.S. Gov't

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H J Ronecker, B Rak. 1987. Genetic organization of insertion element IS2 based on a revised nucleotide sequence.. https://doi.org/10.1016/0378-1119(87)90337-4

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