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BcefI, a new type IIS restriction endonuclease.

P Venetianer·A Orosz
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Abstract

A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a +/- 1 basepair uncertainty. Thus the cleavage and recognition site is as shown below: ACGGC(N)11-13 TGCCG(N)12-14.

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Bacillus cereusBase SequenceCloning, MolecularDNA Restriction EnzymesDeoxyribonucleases, Type II Site-SpecificMolecular Sequence DataPlasmidsSequence Homology, Nucleic AcidJournal Article

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P Venetianer, A Orosz. 1988-04-11. BcefI, a new type IIS restriction endonuclease.. https://doi.org/10.1093/nar%2F16.7.3053

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