Anchored cell analysis/sorting coupled with the scrape-loading/dye-transfer technique to quantify inhibition of gap-junctional intercellular communication in WB-F344 cells by 2,2',4,4',5,5'-hexabromobiphenyl.

M G Evans; M H el-Fouly; J E Trosko; S D Sleight

doi:10.1080/15287398809531159

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Anchored cell analysis/sorting coupled with the scrape-loading/dye-transfer technique to quantify inhibition of gap-junctional intercellular communication in WB-F344 cells by 2,2',4,4',5,5'-hexabromobiphenyl.

M G Evans·M H el-Fouly·J E Trosko·S D Sleight

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Abstract

Inhibition of intercellular communication has been hypothesized to play a role in tumor promotion. The compound 2,2',4,4',5,5'-hexabromobiphenyl (245-HBB) is a tumor promoter in vivo and blocks intercellular communication in vitro. The scrape-loading/dye-transfer (SL/DT) assay was used to assess this in vitro effect at varying concentrations of 245-HBB. The SL/DT technique is based on the intracellular loading of a fluorescent dye, lucifer yellow (LY), and monitoring its transfer into adjacent cells via patent gap junctions. Confluent WB-F344 (rat epithelial) cells were exposed to various noncytolethal concentrations of 245-HBB. Transfer of LY was then quantified with anchored cell analysis/sorting (ACAS 470, Meridian Instruments, Okemos, Mich.). The results indicate an inverse correlation between the degree of fluorescence in secondary LY-recipient cells and the treatment concentration. The coupling of these two new methods of cellular biology provided rapid quantitative analysis of dye transfer in measuring the concentration/response of modulation of gap-junctional permeability in cultured cells.

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Animals Carcinogens Cell Communication In Vitro Techniques Intercellular Junctions Isoquinolines Male Microscopy, Fluorescence Polybrominated Biphenyls Rats Rats, Inbred F344 Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M G Evans, M H el-Fouly, J E Trosko, S D Sleight. 1988. Anchored cell analysis/sorting coupled with the scrape-loading/dye-transfer technique to quantify inhibition of gap-junctional intercellular communication in WB-F344 cells by 2,2',4,4',5,5'-hexabromobiphenyl.. https://doi.org/10.1080/15287398809531159

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