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Evidence that Lactobacillus casei insertion element ISL1 has a narrow host range.

M Shimizu-Kadota·J L Flickinger·B M Chassy
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Abstract

The 1.3-kilobase-pair insertion element ISL1, originally isolated from Lactobacillus casei S-1, was found to have an extremely restricted host range. By DNA-DNA hybrizations performed with Southern transfers by using a cloned internal fragment of ISL1 as a molecular probe, it was found that only 3 of 19 L. casei strains examined contained sequences that hybridized to the ISL1 probe. In two of these, the hybridizing sequences were found on lactose plasmids. No homologous sequences were detected in a survey of 14 other Lactobacillus strains (9 species) and 15 strains of other bacteria (8 genera, 12 species).

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Blotting, SouthernDNA Transposable ElementsLacticaseibacillus caseiSequence Homology, Nucleic AcidSpecies SpecificityJournal Article

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M Shimizu-Kadota, J L Flickinger, B M Chassy. 1988. Evidence that Lactobacillus casei insertion element ISL1 has a narrow host range.. https://doi.org/10.1128/jb.170.10.4976-4978.1988

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