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PubMed · 2851598

Epstein-Barr virus nuclear antigen type 1 binding: electron microscopy.

I Hirsch·J Reischig·O Benada·D Bartsch·B Brichácek·L Boguszaková·V Vonka
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Abstract

Epstein-Barr virus (EBV) nuclear antigen type-1 (EBNA-1) was extracted and purified from Raji cells by chromatography on DNA-Sepharose and Blue-dextran Sepharose. Its complexes with plasmid pM765-10 derived from EBV (strain M-ABA) DNA were visualized by electron microscopy. The criteria of specificity were as follows: (1) preferential binding of EBNA-1 to the ori-P region of pM765-10; (2) specific enlargement of EBV DNA/EBNA-1 complexes with anti-EBNA-1 (IR-3) IgG antibody; and (3) resistance of the resulting EBV DNA/EBNA-1/anti-EBNA-1 antibody complexes to treatment with 1.5 M NaCl. The optimal conditions for the formation of EBV DNA/EBNA-1 complexes were 50 to 150 mM NaCl and pH 6.0. A balanced equilibrium of EBNA-1 and pM765-10 was necessary to achieve both a high yield and specificity of EBV DNA/EBNA-1 complexes.

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Antigens, ViralBinding SitesCell LineCell NucleusDNA, ViralEpstein-Barr Virus Nuclear AntigensHerpesvirus 4, HumanHumansMicroscopy, ElectronPlasmidsJournal Article

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I Hirsch, J Reischig, O Benada, D Bartsch, B Brichácek, L Boguszaková, V Vonka. 1988. Epstein-Barr virus nuclear antigen type 1 binding: electron microscopy.. https://doi.org/10.1016/0166-0934(88)90096-1

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