Structure-independent nucleotide sequence analysis.

D R Mills; F R Kramer

doi:10.1073/pnas.76.5.2232

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PubMed · 287063

Structure-independent nucleotide sequence analysis.

D R Mills·F R Kramer

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Abstract

Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

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Base Sequence Electrophoresis, Polyacrylamide Gel Guanine Nucleotides Hydrogen Bonding Inosine Nucleotides Molecular Weight Nucleic Acid Conformation RNA, Viral Virus Replication Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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D R Mills, F R Kramer. 1979. Structure-independent nucleotide sequence analysis.. https://doi.org/10.1073/pnas.76.5.2232

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