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Preferential glutamine uptake in rat brain synaptic mitochondria.

A Steib·A Rendon·J Mark·J Borg
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Abstract

Glutamine uptake has been studied in purified rat brain mitochondria of synaptic or non-synaptic origin. It was taken up by an active saturable transport mechanism, with an affinity two-times higher in synaptic than in non-synaptic mitochondria (Km = 0.45 and 0.94 mM, respectively). Vmax of uptake was 7-times higher in synaptic mitochondria (Vmax = 9.2 and 1.3 nmol/min per mg protein, respectively). Glutamine transport was found to be inhibited by L-glutamate (IC50 = 0.64 mM) as well as thiol reagents (mersalyl, N-ethylmaleimide). It is suggested that differential uptake of glutamine in mitochondria of synaptic or non-synaptic origin may be a major mechanism in the regulation of the synthesis of the neurotransmitter glutamate.

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AnimalsBiological TransportBrainGlutamatesGlutamic AcidGlutamineIn Vitro TechniquesKineticsMitochondriaRatsRats, Inbred StrainsSulfhydryl ReagentsSynapsesTritiumJournal Article

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A Steib, A Rendon, J Mark, J Borg. 1986-10-20. Preferential glutamine uptake in rat brain synaptic mitochondria.. https://doi.org/10.1016/0014-5793(86)80013-8

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