Splice commitment dictates neuron-specific alternative RNA processing in calcitonin/CGRP gene expression.

S E Leff; R M Evans; M G Rosenfeld

doi:10.1016/0092-8674(87)90202-9

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Splice commitment dictates neuron-specific alternative RNA processing in calcitonin/CGRP gene expression.

S E Leff·R M Evans·M G Rosenfeld

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Abstract

The calcitonin/CGRP gene is a complex transcription unit in which developmentally regulated cell-specific alternative RNA processing results in the production of CGRP mRNA in neurons, and of calcitonin mRNA in thyroid C cells. Alternative poly(A) site selection and exon splicing lead to the production of these alternative mature transcripts. The wild-type and a series of mutated calcitonin/CGRP genes were expressed in heterologous cell types, which produced predominantly calcitonin or CGRP mRNA. The results of these studies suggest that neurons express machinery or a factor that determines a specific alternative splicing pathway and consequently its associated alternative poly(A) site selection. It is hypothesized that a splice commitment regulatory factor might modulate pre-mRNA secondary structure, revealing a cryptic splice site required to generate CGRP mRNA in the brain.

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Animals Calcitonin Calcitonin Gene-Related Peptide Cell Line Genes Introns Models, Genetic Mutation Neurons Neuropeptides Poly A RNA Processing, Post-Transcriptional RNA Splicing RNA, Messenger Transcription, Genetic Transfection Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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S E Leff, R M Evans, M G Rosenfeld. 1987-02-13. Splice commitment dictates neuron-specific alternative RNA processing in calcitonin/CGRP gene expression.. https://doi.org/10.1016/0092-8674(87)90202-9

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