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PubMed · 2880792

A simple DNA diagnostic method for human genetic disorders.

D Rabin·N Dattagupta
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Abstract

A fast, reliable, and simple technique for detecting point mutations in unfractionated human DNA has been developed. Oligonucleotide probes complementary to either sickle- or normal beta-globin DNA are labeled by primer extension and hybridized to DNA applied to nitrocellulose paper in a dot-blot format. A short hybridization time (about 1 h) and low probe concentration (about 1 nM) yield low background and high specificity. Double-blind trials show 100% agreement with restriction fragment length polymorphism (RFLP) analysis of DNA from normal, sickle, and heterozygous subjects.

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Anemia, Sickle CellDNAGenotypeHumansMutationPolymorphism, Restriction Fragment LengthJournal Article

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D Rabin, N Dattagupta. 1987. A simple DNA diagnostic method for human genetic disorders.. https://doi.org/10.1007/bf00591071

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