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Complementary DNA for a human subgroup IV immunoglobulin lambda-chain.

N Yamasaki·S Komori·T Watanabe
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Abstract

We have cloned a cDNA (p3C4 lambda 5) encoding a human immunoglobulin lambda-chain of human-mouse heterohybridoma, H6-3C4, which produces a human monoclonal antibody against human sperm surface antigen. Amino acid sequence deduced from the nucleotide sequence of V lambda (V lambda 3C4) of cloned cDNA was closely related to that of SHV lambda protein, a member of subgroup IV. Southern blot analysis of human genomic DNAs by using V lambda 3C4 as a probe detected at least 8 cross-hybridizing members of subgroup V lambda IV. Restriction enzyme fragment polymorphism (RFLP) appeared to be present in normal individuals with V lambda 3C4 gene.

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Amino Acid SequenceBase SequenceCloning, MolecularDNAElectrophoresis, Agar GelGenes, MHC Class IIHumansImmunoglobulin lambda-ChainsMolecular Sequence DataPolymorphism, Restriction Fragment LengthJournal ArticleResearch Support, Non-U.S. Gov't

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N Yamasaki, S Komori, T Watanabe. 1987. Complementary DNA for a human subgroup IV immunoglobulin lambda-chain.. https://doi.org/10.1016/0161-5890(87)90010-1

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