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Immunoregulatory functions of paf-acether. II. Decrease of CD2 and CD3 antigen expression.

E Vivier·P Salem·A Dulioust·D Praseuth·P Metezeau·J Benveniste·Y Thomas
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Abstract

Paf-acether (platelet-activating factor) is a phospholipid initially described as a potent platelet-aggregating compound. It is produced by numerous cell types and is now considered as an important mediator of cell-cell interactions. The effect of paf-acether on the expression of CD2 and CD3, two human T cell surface glycoproteins, was investigated by indirect immunofluorescence and flow cytometry. Paf-acether partially down-regulated, in a time- and dose-dependent manner, CD2 and CD3 but not HLA class I antigen expression on peripheral human T cells and Jurkat cells. Lysophosphatidylcholine, a phospholipid closely related to paf-acether, had no detectable modulatory effect on CD2 and CD3 expression. In addition to CD2/CD3 modulation, paf-acether markedly inhibited T cell proliferative response not only to phytohemagglutinin or concanavalin A but also to anti-CD3 or a stimulatory combination of anti-CD2 monoclonal antibodies. These data demonstrate for the first time that lipid mediators such as paf-acether might be involved in the regulation of the expression of cell surface glycoproteins that are essential in the execution of T cell function.

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Antibodies, MonoclonalAntigens, Differentiation, T-LymphocyteCD2 AntigensCD3 ComplexCarrier ProteinsConcanavalin ADepression, ChemicalGene Expression RegulationHumansLymphocyte ActivationLysophosphatidylcholinesPhytohemagglutininsPlatelet Activating FactorReceptors, ImmunologicT-LymphocytesTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov't

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E Vivier, P Salem, A Dulioust, D Praseuth, P Metezeau, J Benveniste, Y Thomas. 1988. Immunoregulatory functions of paf-acether. II. Decrease of CD2 and CD3 antigen expression.. https://doi.org/10.1002/eji.1830180316

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