Neuroimmunological electron microscopy with microwave-accelerated fixation.

P J Armati; J D Pollard; D Van Reyk; L Van der Lubbe

doi:10.1016/0022-1759(88)90113-5

PubMed · 2897992

Neuroimmunological electron microscopy with microwave-accelerated fixation.

P J Armati·J D Pollard·D Van Reyk·L Van der Lubbe

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Abstract

Immunoelectron microscopy is an important tool used to determine the precise location of immune complexes. Standard concentrations of glutaraldehyde destroy these complexes. This paper describes a method in which the period of glutaraldehyde fixation is shortened by concomitant microwave treatment. Using 1.25% glutaraldehyde and microwave fixation ideal preservation and demonstration of MHC class I antigen on Schwann cells was obtained by the peroxidase method.

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Aldehydes Animals Antigen-Antibody Complex Endosomes Fixatives Ganglia, Spinal Glutaral Microscopy, Electron Microwaves Rats Rats, Inbred Strains Schwann Cells Journal Article Research Support, Non-U.S. Gov't

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P J Armati, J D Pollard, D Van Reyk, L Van der Lubbe. 1988-06-13. Neuroimmunological electron microscopy with microwave-accelerated fixation.. https://doi.org/10.1016/0022-1759(88)90113-5

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Neuroimmunological electron microscopy with microwave-accelerated fixation.

Abstract

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