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PubMed · 2903694

Haplotype analysis of the human beta-globin gene complex using multiple locus specific oligonucleotide probes.

G Nozari·S Rahbar·R B Wallace
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Abstract

Three oligonucleotide probes complementary to specific DNA sequences of the six human globin genes (epsilon, G gamma, A gamma, psi beta, delta, beta) were synthesized. The oligonucleotides were used either singly or in combination as hybridization probes to determine the haplotype of the human beta-globin gene cluster employing the four conventionally used restriction endonucleases HincII, HindIII, AvaII, and BamHI, in addition to HpaI. Polymorphism in the epsilon- and psi beta-genes (HincII) can be simultaneously determined with a single probe mixture. One of the probes complementary to both the psi beta- and gamma-genes is useful for determining both HindIII and HincII polymorphisms. The advantages of these probes relative to conventional cDNA probes are discussed.

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Base SequenceCloning, MolecularDNA ProbesDNA Restriction EnzymesGlobinsHaplotypesHumansHydrolysisNucleic Acid HybridizationOligonucleotide ProbesPolymorphism, Restriction Fragment LengthJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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G Nozari, S Rahbar, R B Wallace. 1988. Haplotype analysis of the human beta-globin gene complex using multiple locus specific oligonucleotide probes.. https://doi.org/10.1016/0003-2697(88)90429-0

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