Rapid DNA purification for restriction fragment length polymorphism analysis.

B Lindblom; G Holmlund

doi:10.1016/0735-0651(88)90003-9

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Rapid DNA purification for restriction fragment length polymorphism analysis.

B Lindblom·G Holmlund

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Abstract

This paper describes a method for isolation of DNA from blood samples involving a rapid chemical disintegration of proteins with 8 M urea and with a minimum of exposure to phenol. The DNA is further desalted and purified on Sephadex G-25 prepacked disposable columns. DNA isolated in this way was pure enough to be immediately cleaved by restriction enzymes.

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Autoradiography Blotting, Southern DNA DNA Restriction Enzymes Electrophoresis, Agar Gel Humans Nucleic Acid Hybridization Polymorphism, Genetic Polymorphism, Restriction Fragment Length Journal Article Research Support, Non-U.S. Gov't

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B Lindblom, G Holmlund. Rapid DNA purification for restriction fragment length polymorphism analysis.. https://doi.org/10.1016/0735-0651(88)90003-9

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