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PubMed · 2904811

H2O2 activates CD11b/CD18-dependent cell adhesion.

G Skoglund·I Cotgreave·J Rincon·M Patarroyo·M Ingelman-Sundberg
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Abstract

Treatment of monoblastoid U-937 cells with low concentrations of H2O2 caused adhesion of the cells to plastic. The H2O2 induced adhesion was rapid with a t1/2 of congruent to 6 min and was optimally stimulated by 100 microM H2O2 with an ED50 of congruent to 50 microM. The response to H2O2 closely resembled the adhesive response of U-937 cells to phorbol esters in its time dependency, requirement for extracellular Mg2+ and inhibition by cytochalasin B as well as inhibition by monoclonal antibodies against the leucocyte adhesion molecules CD11b and CD18. Phorbol ester treatment of U-937 cells stimulated the phosphorylation of at least three endogenous substrates, pp28, pp34 and pp43, of which pp28 and pp43 also responded to H2O2-treatment with increased 32P-incorporation. The results suggest that H2O2 might be a physiological modulator of leucocyte adhesion, possibly operating by activating protein kinase C.

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Antibodies, MonoclonalAntigens, DifferentiationCD18 AntigensCalciumCell AdhesionCell LineCytochalasin BHumansHydrogen PeroxideMacrophage-1 AntigenMagnesiumMembrane GlycoproteinsPhorbol 12,13-DibutyratePhosphoproteinsPhosphorylationJournal ArticleResearch Support, Non-U.S. Gov't

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G Skoglund, I Cotgreave, J Rincon, M Patarroyo, M Ingelman-Sundberg. 1988-12-15. H2O2 activates CD11b/CD18-dependent cell adhesion.. https://doi.org/10.1016/s0006-291x(88)80269-9

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