Complement C9 is inserted into membranes in a globular conformation.

D Marazziti; J P Luzio; K K Stanley

doi:10.1016/0014-5793(89)80159-0

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Complement C9 is inserted into membranes in a globular conformation.

D Marazziti·J P Luzio·K K Stanley

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Abstract

Complement component C9 undergoes a major conformational change during its insertion into a biological membrane from a globular to an extended form. At 0 degrees C a single C9 binds but a membrane attack complex (MAC) is not formed. We show that the C9 bound at 0 degrees is accessible to the intracellular space and sensitive to trypsin digestion, suggesting that C9 inserts in its globular state and requires an elevated temperature in order to change conformation.

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Animals Antibodies, Monoclonal Binding Sites Cell Membrane Complement C9 Erythrocyte Membrane In Vitro Techniques Membrane Proteins Precipitin Tests Protein Conformation Rabbits Thermodynamics Trypsin Journal Article Research Support, Non-U.S. Gov't

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D Marazziti, J P Luzio, K K Stanley. 1989-01-30. Complement C9 is inserted into membranes in a globular conformation.. https://doi.org/10.1016/0014-5793(89)80159-0

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