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PubMed · 2931321

[W-mutagenesis in the bisulfite-treated lambda phage].

L V Konevega·V L Kalinin
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Abstract

Survival of phage lambda cI857 inactivated by bisulfite (pH 5.6, 37 degrees C) is higher (the dose modification factor approx. 1.2) and frequency of bisulfite-induced c-mutations 2-4-fold lower on the lawn of the wild-type strain ung+, as compared to ung-1 mutant deficient in uracil-DNA glycosylase. Irradiation of host cells by a moderate UV dose inducing SOS repair system enhances the frequency of bisulfite-induced c-mutations 2-3-fold in the wild-type (ung+) host, but not in the ung-1 mutant. It is suggested that W-mutagenesis in bisulfite-treated lambda phage in the ung+ cells is due to SOS repair of apyrimidinic sites which are produced during excision of uracil residues, the products of cytosine deamination.

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Bacteriophage lambdaDNA GlycosylasesDNA RepairDNA, ViralMutationN-Glycosyl HydrolasesSulfitesUltraviolet RaysUracil-DNA GlycosidaseComparative StudyEnglish AbstractJournal Article

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L V Konevega, V L Kalinin. 1985. [W-mutagenesis in the bisulfite-treated lambda phage].. https://pubmed.ncbi.nlm.nih.gov/2931321/

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