Electron microscopic localization of cell-surface markers in tissue sections using monoclonal and gold-conjugated antibodies.

N Rooney; C Day; T Gray; J C Underwood

doi:10.1002/path.1711480106

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Electron microscopic localization of cell-surface markers in tissue sections using monoclonal and gold-conjugated antibodies.

N Rooney·C Day·T Gray·J C Underwood

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Abstract

We describe a method for the ultrastructural localization of surface markers on cells in intact tissue sections. Using a primary monoclonal antibody to a surface marker followed by a second stage gold-labelled antibody, surface antigens can be specifically localized. This enables cellular interactions to be studied in solid tissues.

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Adenocarcinoma Antibodies Antibodies, Monoclonal Antigens, Surface Breast Neoplasms Female Gold Hodgkin Disease Humans Lymph Nodes Microscopy, Electron T-Lymphocytes, Cytotoxic T-Lymphocytes, Helper-Inducer Journal Article Research Support, Non-U.S. Gov't

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N Rooney, C Day, T Gray, J C Underwood. 1986. Electron microscopic localization of cell-surface markers in tissue sections using monoclonal and gold-conjugated antibodies.. https://doi.org/10.1002/path.1711480106

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