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PubMed · 293659

Covalent association of protein with replicative form DNA of parvovirus H-1.

D Revie·B Y Tseng·R H Grafstrom·M Goulian
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Abstract

The double-stranded replicative form (RF) DNA of the autonomous parvovirus H-1 can be isolated from infected cells in a covalent complex with protein. The protein is present on most or all of the RF DNA, including actively replicating molecules, and is associated with the 5'-terminal endonuclease Hae III fragments of both the viral and complementary strands of RF. The size of the protein is estimated to be 60,000-70,000 daltons from its effect on buoyant density of DNA. DNA with covalently bound protein has not been found in H-1 virions.

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Centrifugation, IsopycnicDNA ReplicationDNA, ViralDeoxyribonucleoproteinsMolecular WeightNucleoproteinsParvoviridaeViral ProteinsVirus ReplicationJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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D Revie, B Y Tseng, R H Grafstrom, M Goulian. 1979. Covalent association of protein with replicative form DNA of parvovirus H-1.. https://doi.org/10.1073/pnas.76.11.5539

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