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Anisotropic molecular motion on cell surfaces.

B A Smith·W R Clark·H M McConnell
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Abstract

Photobleaching in a two-dimensional periodic pattern has been used to characterize the motion of succinylconcanavalin A receptors on the surfaces of mouse fibroblasts. These receptors were found to exhibit anisotropic two-dimensional diffusion in adherent cells having parallel stress fibers. Diffusion coefficients of the order of 10(-11) cm2/sec and 10(-12) cm2/sec were measured for diffusive motions parallel and perpendicular to these fibers. The determination of the anisotropic motion used a two-dimensional Fourier analysis. A study was made of the effect of changes in cell shape on the photobleach pattern.

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AnimalsCell MovementDiffusionFibroblastsFourier AnalysisMembrane FluidityMicePhotochemistryReceptors, Concanavalin ATemperatureJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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B A Smith, W R Clark, H M McConnell. 1979. Anisotropic molecular motion on cell surfaces.. https://doi.org/10.1073/pnas.76.11.5641

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