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Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.

C T Lutz·W C Hollifield·B Seed·J M Davie·H V Huang
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Abstract

The Syrinx 2A phage and pi AN13 plasmid were designed for screening of DNA libraries by homologous recombination in vivo. Syrinx 2A carries multiple cloning sites and a recently identified lambda gene, rap (recombination adept with plasmid), required for efficient phage-plasmid recombination. We describe a rapid, reliable, and technically easy method to screen Syrinx 2A libraries, expand the resulting phage-plasmid cointegrates, and subclone plasmid in as little as 2 days. Recombination screening allows one specific member of a closely related multigene family to be isolated selectively.

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Bacteriophage lambdaCloning, MolecularDNA, RecombinantGenetic VectorsImmunoglobulin Variable RegionImmunoglobulin kappa-ChainsMultigene FamilyPlasmidsRecombination, GeneticJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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C T Lutz, W C Hollifield, B Seed, J M Davie, H V Huang. 1987. Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.. https://doi.org/10.1073/pnas.84.13.4379

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